《植物生理学报》 2015, 51(4): 424-428

干旱胁迫下番茄叶片碳酸酐酶活性的变化

孙卫红^1,*, 吴秋霞¹, 温新宇¹, 何华纲¹, 吴沿友², 丁佳俊¹, 封丽娜¹

江苏大学¹食品与生物工程学院; ²农业工程研究院, 江苏镇江212013

通信作者：孙卫红；E-mail: weihongsun2009@163.com；Tel: 0511-88797059

摘　要：

碳酸酐酶(carbonic anhydrase, CA)催化CO₂的可逆水合反应, 是参与CO₂传导而进入羧化位点的重要光合酶, 其活性高低对植物水分利用及光合作用有较大影响。为了研究干旱胁迫下番茄CA表达活性变化对植株光合作用及水分利用的影响, 本文利用Northern杂交检测番茄叶片的胞质CA基因在干旱胁迫下的表达; 测定干旱胁迫下番茄叶片CA活性、光合速率和叶片水势变化。结果表明, 番茄叶片胞质CA基因受干旱胁迫诱导; CA活性随着干旱胁迫时间延长而发生由低至高再回落的变化, 且变化规律基本与CA基因相似。番茄叶片的净光合速率和水势随着干旱程度和处理时间延长而降低。在75%的土壤相对水分含量条件下, 植株的CA活性、光合速率及水势变化不明显。可见番茄叶水势和光合速率与CA表达活性之间存在一定的变化趋势相似性。

关键词：干旱胁迫; 番茄叶片; 碳酸酐酶活性

收稿：2014-11-06   修定：2015-03-14

资助：江苏省自然基金面上项目(BK20130503)、江苏省博士后基金项目(1102132C)和江苏高校优势学科建设工程资助项目。

Changes of Carbonic Anhydrase Activities in Tomato Leaves under Drought Stress

SUN Wei-Hong^1,*, WU Qiu-Xia¹, WEN Xin-Yu¹, HE Hua-Gang¹, WU Yan-You², DING Jia-Jun¹, FENG Li-Na¹

¹School of Food and Biological Engineering; ²Institute of Agricultural Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China

Corresponding author: SUN Wei-Hong; E-mail: weihongsun2009@163.com; Tel: 0511-88797059

Abstract:

Carbonic anhydrase (CA) catalyzes the reversible hydration of CO₂, and it is an important photosynthetic enzyme which take part in the CO₂ conduction and get into the carboxylation site. The CA activity has a great effect on photosynthesis and water use efficiency. In order to study the effect of expression of CA gene on the plant photosynthesis and plant water potential in tomato (Lycopersicon esculentum), the expression of cytosolic CA gene in tomato leaves under drought stress were determined by Northern blotting. And the CA enzyme activity, photosynthetic rate and water potential in tomato leaves under drought stress were also determined. The results showed that the expression of cytosolic CA gene in tomato leaves was induced by drought stress. With the time of drought stress, the CA activity increased and then fell, and the changes were basically similar to that of CA gene expression. Net photosynthetic rate and water potential of tomato leaves decreased with the degree of drought and the treatment time. However, the CA activity, net photosynthetic rate and water potential changed slightly with the soil relative water content of 75%. The results indicated that there was a certain corresponding relationship between the changes of water potential and photosynthetic rate in tomato leaves and the change of CA activity.

Key words: drought stress; tomato (Lycopersicon esculentum) leaves; CA activity